Proteins are responsible for enacting the function of genes in the body. Studying proteins and their modifications are essential to elucidating disease progression or identifying possible drug targets. Better understanding of biology at the protein level has been a growing field. New technologies are becoming more sensitive and automated to accommodate the complexities of cells, tissues and organisms being studied.
The application of proteomic technologies for clinical medicine, or clinical proteomics, relies on rapid protein identification with a high level of consistency. Clinical proteomics involves the application of studying specific proteomes and identifying their unique characteristics responsible for diagnosis, prognosis and therapeutic prediction of diseases. These unique characteristics or biomarkers are biological molecules found in blood, other bodily fluids and tissues. These biomarkers are signals of normal or abnormal processes, or of a condition or a disease. Effective detection and treatment lie in the understanding and discovery of these markers. Measuring what is happening in a patient in real time can come with finding these tell-tale proteins.
At IMCS, our goal is to develop tools in proteomics that accelerate the understanding of protein functions and discovery biomarkers. IMCStips provide scientists with a variety of tools and methods to quickly prepare samples for a variety of downstream applications. IMCStips enable rapid quantification of low abundant proteins involved in critical cellular events. Consistent performance, high reproducibility and high recoveries make IMCStips ideal for a wide range of protein purification applications.
IMCStips remove detergents and salts from complex protein digest. IMCStips can achieve >90% peptide recoveries. IMCS has optimized protocols for desalting and enrichment methods. Extraction methods follow a simple bind-wash-elute protocol.
Contact us to learn more about available automated scripts.
Phosphorylation events are key signal transduction mechanisms within the eukaryotic cells and further understanding of such signaling pathways can unravel crucial molecular signatures of various human diseases. The global deep profiling of phosphospeptides has been successfully optimized in the procedures of enrichment, mass spectrometry, and data analysis. Mass spectrometry is a key tool for identifying sites of protein phosphorylation and quantifying phosphorylation changes. The low relative abundance of phosphorylation modifications in complex protein samples makes enrichment essential for successful MS analysis of phosphopeptides. Our scientists have developed high-throughput methods using TiO2 and ZrO2 IMCStips to improve efficiency for phosphopeptide enrichment. Contact us to learn more about our phosphopeptide purification methods.